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Image Search Results
Journal: Comprehensive Physiology
Article Title: Porcine Fetal Hypothyroidism Induces Temporal and Tissue‐Specific Alterations in the Insulin‐Like Growth Factor System
doi: 10.1002/cph4.70028
Figure Lengend Snippet: IGFBP levels in fetal serum. (A) Example Western Ligand Blot showing human recombinant protein standards in the left lane, with the right lane showing the six bands detected in fetal porcine serum. Relative density of (B) a 26 kDa IGFBP, (C) a 28 kDa IGFBP, (D) IGFBP2, (E) the 40 kDa IGFBP3, and (F) IGFBP4 in fetal porcine serum derived from fetuses ( n = 11–12/group) at various gestational timepoints following 21 days of maternal CON or MMI treatment. Relative density was calculated relative to a consistent reference sample run on each gel, with unique letter superscripts denoting statistically significant ( p < 0.05) changes in density in the CON group over time, and statistical differences between the CON and MMI group within each timepoint denoted as * p < 0.05, ** p < 0.01, and *** p < 0.001. Data are presented as the estimated marginal means with 95% confidence intervals, as extracted from a linear model including gestational day, treatment group, and the interaction.
Article Snippet: In addition to samples of interest, each blot contained a lane of pooled
Techniques: Western Blot, Recombinant, Derivative Assay